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Published online
doi:10.1083/jcb.200801153
The Journal of Cell Biology, Vol. 183, No. 1, 63-75
The Rockefeller University Press, 0021-9525 $30.00
© Royou et al.
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Article

Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation



Anne Royou, Derek McCusker, Douglas R. Kellogg, and William Sullivan

Department of Molecular Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064

Correspondence to Anne Royou: royou{at}biology.ucsc.edu

Entry into mitosis is characterized by a dramatic remodeling of nuclear and cytoplasmic compartments. These changes are driven by cyclin-dependent kinase 1 (CDK1) activity, yet how cytoplasmic and nuclear CDK1 activities are coordinated is unclear. We injected cyclin B (CycB) into Drosophila melanogaster embryos during interphase of syncytial cycles and monitored effects on cytoplasmic and nuclear mitotic events. In untreated embryos or embryos arrested in interphase with a protein synthesis inhibitor, injection of CycB accelerates nuclear envelope breakdown and mitotic remodeling of the cytoskeleton. Upon activation of the Grapes(checkpoint kinase 1) (Grp(Chk1))-dependent S-phase checkpoint, increased levels of CycB drives cytoplasmic but not nuclear mitotic events. Grp(Chk1) prevents nuclear CDK1 activation by delaying CycB nuclear accumulation through Wee1-dependent and independent mechanisms.

Abbreviations used in this paper: Aph, aphidicolin; AVD, average deviation; Chk1, checkpoint kinase 1; CHX, cycloheximide; CycB, cyclin B; Grp, Grapes; MTOC, microtubule-organizing center; NEB, nuclear envelope breakdown; NEF, nuclear envelope formation; RLC, regulatory light chain.

© 2008 Royou et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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