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Published online
doi:10.1083/jcb.200805185
The Journal of Cell Biology, Vol. 183, No. 2, 187-194
The Rockefeller University Press, 0021-9525 $30.00
© Yamamoto et al.
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SPDL-1 functions as a kinetochore receptor for MDF-1 in Caenorhabditis elegans



Takaharu G. Yamamoto1, Sonoko Watanabe1, Anthony Essex2,3, and Risa Kitagawa1

1 Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, TN 38105
2 Ludwig Institute for Cancer Research/Department of Cellular and Molecular Medicine, 3 Biomedical Sciences Graduate Program, School of Medicine, University of California, San Diego, La Jolla, CA 92093

Correspondence to Risa Kitagawa: risa.kitagawa{at}stjude.org

The spindle assembly checkpoint (SAC) ensures faithful chromosome segregation by delaying anaphase onset until all sister kinetochores are attached to bipolar spindles. An RNA interference screen for synthetic genetic interactors with a conserved SAC gene, san-1/MAD3, identified spdl-1, a Caenorhabditis elegans homologue of Spindly. SPDL-1 protein localizes to the kinetochore from prometaphase to metaphase, and this depends on KNL-1, a highly conserved kinetochore protein, and CZW-1/ZW10, a component of the ROD–ZW10–ZWILCH complex. In two-cell–stage embryos harboring abnormal monopolar spindles, SPDL-1 is required to induce the SAC-dependent mitotic delay and localizes the SAC protein MDF-1/MAD1 to the kinetochore facing away from the spindle pole. In addition, SPDL-1 coimmunoprecipitates with MDF-1/MAD1 in vivo. These results suggest that SPDL-1 functions in a kinetochore receptor of MDF-1/MAD1 to induce SAC function.

Abbreviations used in this paper: APC/C, anaphase-promoting complex/cyclosome; dsRNA, double-stranded RNA; NEBD, nuclear envelope breakdown; RZZ, ROD–ZW10–ZWILCH; SAC, spindle assembly checkpoint.

© 2008 Yamamoto et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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