Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 2831K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Buchan, J. R. Articles by Parker, R. Search for Related Content PubMed PubMed Citation Articles by Buchan, J. R. Articles by Parker, R. Social Bookmarking                            What's this?

P bodies promote stress granule assembly in Saccharomyces cerevisiae

Department of Molecular and Cellular Biology, and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721

Correspondence to Roy Parker: rrparker{at}u.arizona.edu

Recent results indicate that nontranslating mRNAs in eukaryotic cells exist in distinct biochemical states that accumulate in P bodies and stress granules, although the nature of interactions between these particles is unknown. We demonstrate in Saccharomyces cerevisiae that RNA granules with similar protein composition and assembly mechanisms as mammalian stress granules form during glucose deprivation. Stress granule assembly is dependent on P-body formation, whereas P-body assembly is independent of stress granule formation. This suggests that stress granules primarily form from mRNPs in preexisting P bodies, which is also supported by the kinetics of P-body and stress granule formation both in yeast and mammalian cells. These observations argue that P bodies are important sites for decisions of mRNA fate and that stress granules, at least in yeast, primarily represent pools of mRNAs stalled in the process of reentry into translation from P bodies.

© 2008 Buchan et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Pomeranz, M. C., Hah, C., Lin, P.-C., Kang, S. G., Finer, J. J., Blackshear, P. J., Jang, J.-C. (2010). The Arabidopsis Tandem Zinc Finger Protein AtTZF1 Traffics between the Nucleus and Cytoplasmic Foci and Binds Both DNA and RNA. Plant Physiol. 152: 151-165 [Abstract] [Full Text]  
• Suzuki, Y., Minami, M., Suzuki, M., Abe, K., Zenno, S., Tsujimoto, M., Matsumoto, K., Minami, Y. (2009). The Hsp90 Inhibitor Geldanamycin Abrogates Colocalization of eIF4E and eIF4E-Transporter into Stress Granules and Association of eIF4E with eIF4G. J. Biol. Chem. 284: 35597-35604 [Abstract] [Full Text]  
• Loschi, M., Leishman, C. C., Berardone, N., Boccaccio, G. L. (2009). Dynein and kinesin regulate stress-granule and P-body dynamics. J. Cell Sci. 122: 3973-3982 [Abstract] [Full Text]  
• Souquere, S., Mollet, S., Kress, M., Dautry, F., Pierron, G., Weil, D. (2009). Unravelling the ultrastructure of stress granules and associated P-bodies in human cells. J. Cell Sci. 122: 3619-3626 [Abstract] [Full Text]  
• Farny, N. G., Kedersha, N. L., Silver, P. A. (2009). Metazoan stress granule assembly is mediated by P-eIF2{alpha}-dependent and -independent mechanisms. RNA 15: 1814-1821 [Abstract] [Full Text]  
• Chang, W.-L., Tarn, W.-Y. (2009). A role for transportin in deposition of TTP to cytoplasmic RNA granules and mRNA decay. Nucleic Acids Res 37: 6600-6612 [Abstract] [Full Text]  
• Xu, J., Chua, N.-H. (2009). Arabidopsis Decapping 5 Is Required for mRNA Decapping, P-Body Formation, and Translational Repression during Postembryonic Development. Plant Cell 21: 3270-3279 [Abstract] [Full Text]  
• Grousl, T., Ivanov, P., Frydlova, I., Vasicova, P., Janda, F., Vojtova, J., Malinska, K., Malcova, I., Novakova, L., Janoskova, D., Valasek, L., Hasek, J. (2009). Robust heat shock induces eIF2{alpha}-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae. J. Cell Sci. 122: 2078-2088 [Abstract] [Full Text]  
• Balagopal, V., Parker, R. (2009). Stm1 Modulates mRNA Decay and Dhh1 Function in Saccharomyces cerevisiae. Genetics 181: 93-103 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents