JCB logo
ReproCELL
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online November 10, 2008
doi:10.1083/jcb.200808018
The Journal of Cell Biology, Vol. 183, No. 4, 607-615
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Sinka et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 3771K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sinka, R.
Right arrow Articles by Munro, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sinka, R.
Right arrow Articles by Munro, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

Golgi coiled-coil proteins contain multiple binding sites for Rab family G proteins



Rita Sinka1, Alison K. Gillingham1, Vangelis Kondylis2,3, and Sean Munro1

1 Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, England, UK
2 Cell Microscopy Center Department of Cell Biology, and 3 Institute of Biomembranes, University Medical Center, Utrecht 3584CX, Netherlands

Correspondence to S. Munro: sean{at}mrc-lmb.cam.ac.uk

Vesicles and other carriers destined for the Golgi apparatus must be guided to the correct cisternae. Golgins, long coiled-coil proteins that localize to particular Golgi subdomains via their C termini, are candidate regulators of vesicle sorting. In this study, we report that the GRIP domain golgins, whose C termini bind the Arf-like 1 G protein on the trans-Golgi, can also bind four members of the Rab family of G proteins. The Rab2-, Rab6-, Rab19-, and Rab30-binding sites are within the coiled-coil regions that are not required for Golgi targeting. Binding sites for two of these Rabs are also present on two coiled-coil proteins of the cis-Golgi, the Drosophila melanogaster orthologues of GM130 and GMAP-210. We suggest an integrated model for a tentacular Golgi in which coiled-coil proteins surround the Golgi to capture and retain Rab-containing membranes, excluding other structures such as ribosomes. Binding sites for diverse Rabs could ensure that incoming carriers are captured on first contact and moved to their correct destination within the stack.

Abbreviations used in this paper: Arl1, Arf-like 1; BicD, BicaudalD; dGMAP, Drosophila Golgi microtubule-associated protein; dsRNA, double-stranded RNA.

© 2008 Sinka et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents