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A kinesin-13 mutant catalytically depolymerizes microtubules in ADP

¹ Deptartment of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA 98107
² Center for Cell Dynamics, Friday Harbor Laboratories, Friday Harbor, WA 98250

Correspondence to Linda Wordeman: worde{at}u.washington.edu

The kinesin-13 motor protein family members drive the removal of tubulin from microtubules (MTs) to promote MT turnover. A point mutation of the kinesin-13 family member mitotic centromere-associated kinesin/Kif2C (E491A) isolates the tubulin-removal conformation of the motor, and appears distinct from all previously described kinesin-13 conformations derived from nucleotide analogues. The E491A mutant removes tubulin dimers from stabilized MTs stoichiometrically in adenosine triphosphate (ATP) but is unable to efficiently release from detached tubulin dimers to recycle catalytically. Only in adenosine diphosphate (ADP) can the mutant catalytically remove tubulin dimers from stabilized MTs because the affinity of the mutant for detached tubulin dimers in ADP is low relative to lattice-bound tubulin. Thus, the motor can regenerate for further cycles of disassembly. Using the mutant, we show that release of tubulin by kinesin-13 motors occurs at the transition state for ATP hydrolysis, which illustrates a significant divergence in their coupling to ATP turnover relative to motile kinesins.

© 2008 Wagenbach et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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• Peris, L., Wagenbach, M., Lafanechere, L., Brocard, J., Moore, A. T., Kozielski, F., Job, D., Wordeman, L., Andrieux, A. (2009). Motor-dependent microtubule disassembly driven by tubulin tyrosination. JCB 185: 1159-1166 [Abstract] [Full Text]  
• Mulder, A. M., Glavis-Bloom, A., Moores, C. A., Wagenbach, M., Carragher, B., Wordeman, L., Milligan, R. A. (2009). A new model for binding of kinesin 13 to curved microtubule protofilaments. JCB 185: 51-57 [Abstract] [Full Text]  

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