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Published online November 10, 2008
doi:10.1083/jcb.200806065
The Journal of Cell Biology, Vol. 183, No. 4, 641-651
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Hiraga et al.
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Article

Histone H3 lysine 56 acetylation by Rtt109 is crucial for chromosome positioning



Shin-ichiro Hiraga, Sotirios Botsios, and Anne D. Donaldson

Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK

Correspondence to Anne D. Donaldson: a.d.donaldson{at}abdn.ac.uk.

Correct intranuclear organization of chromosomes is crucial for many genome functions, but the mechanisms that position chromatin are not well understood. We used a layered screen to identify Saccharomyces cerevisiae mutants defective in telomere localization to the nuclear periphery. We find that events in S phase are crucial for correct telomere localization. In particular, the histone chaperone Asf1 functions in telomere peripheral positioning. Asf1 stimulates acetylation of histone H3 lysine 56 (H3K56) by the histone acetyltransferase Rtt109. Analysis of rtt109{Delta} and H3K56 mutants suggests that the acetylation/deacetylation cycle of the H3K56 residue is required for proper telomere localization. The function of H3K56 acetylation in localizing chromosome domains is not confined to telomeres because deletion of RTT109 also prevents the correct peripheral localization of a newly identified S. cerevisiae "chromosome-organizing clamp" locus. Because chromosome positioning is subject to epigenetic inheritance, H3K56 acetylation may mediate correct chromosome localization by facilitating accurate transmission of chromatin status during DNA replication.

Abbreviations used in this paper: COC, chromosome-organizing clamp; ETC, extra TFIIIC; H3–H4, histone H3–H4 complex; H3K56, histone H3 lysine 56; NPC, nuclear pore complex; ORF, open reading frame; RLC, replication factor C–like complex; TFIIIC, transcription factor IIIC; WT, wild-type; XIV-L, XIV-left.

© 2008 Hiraga et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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