JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online December 1, 2008
doi:10.1083/jcb.200806088
The Journal of Cell Biology, Vol. 183, No. 5, 881-892
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Gu et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 7159K)
Right arrow PDF+supp data (17228K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gu, M.
Right arrow Articles by Jorgensen, E. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Gu, M.
Right arrow Articles by Jorgensen, E. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

µ2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans



Mingyu Gu1, Kim Schuske1, Shigeki Watanabe1, Qiang Liu1, Paul Baum2,3, Gian Garriga2, and Erik M. Jorgensen1

1 Howard Hughes Medical Institute and Department of Biology, University of Utah, Salt Lake City, UT 84112
2 Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720
3 Division of Experimental Medicine, Department of Medicine, San Francisco General Hospital, University of California, San Francisco, San Francisco, CA 94110

Correspondence to Erik M. Jorgensen: jorgensen{at}biology.utah.edu

Synaptic vesicles must be recycled to sustain neurotransmission, in large part via clathrin-mediated endocytosis. Clathrin is recruited to endocytic sites on the plasma membrane by the AP2 adaptor complex. The medium subunit (µ2) of AP2 binds to cargo proteins and phosphatidylinositol-4,5-bisphosphate on the cell surface. Here, we characterize the apm-2 gene (also called dpy-23), which encodes the only µ2 subunit in the nematode Caenorhabditis elegans. APM-2 is highly expressed in the nervous system and is localized to synapses; yet specific loss of APM-2 in neurons does not affect locomotion. In apm-2 mutants, clathrin is mislocalized at synapses, and synaptic vesicle numbers and evoked responses are reduced to 60 and 65%, respectively. Collectively, these data suggest AP2 µ2 facilitates but is not essential for synaptic vesicle recycling.

Abbreviations used in this paper: CHC, clathrin heavy chain; ePSC, evoked postsynaptic current; mPSC, miniature postsynaptic current; ORF, open reading frame.

© 2008 Gu et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents