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Published online November 24, 2008
doi:10.1083/jcb.200808050
The Journal of Cell Biology, Vol. 183, No. 5, 923-932
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Bui et al.
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Article

Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella



Khanh Huy Bui1, Hitoshi Sakakibara2, Tandis Movassagh1, Kazuhiro Oiwa2,3, and Takashi Ishikawa1

1 Department of Biology, Eidgenössische Technische Hochschule Zürich, CH8093 Zurich, Switzerland
2 Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Iwaoka, Kobe 6512492, Japan
3 Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo 6781297, Japan

Correspondence to Takashi Ishikawa: takashi.ishikawa{at}mol.biol.ethz.ch

The inner dynein arm regulates axonemal bending motion in eukaryotes. We used cryo-electron tomography to reconstruct the three-dimensional structure of inner dynein arms from Chlamydomonas reinhardtii. All the eight different heavy chains were identified in one 96-nm periodic repeat, as expected from previous biochemical studies. Based on mutants, we identified the positions of the AAA rings and the N-terminal tails of all the eight heavy chains. The dynein f dimer is located close to the surface of the A-microtubule, whereas the other six heavy chain rings are roughly colinear at a larger distance to form three dyads. Each dyad consists of two heavy chains and has a corresponding radial spoke or a similar feature. In each of the six heavy chains (dynein a, b, c, d, e, and g), the N-terminal tail extends from the distal side of the ring. To interact with the B-microtubule through stalks, the inner-arm dyneins must have either different handedness or, more probably, the opposite orientation of the AAA rings compared with the outer-arm dyneins.

Abbreviations used in this paper: DRC, dynein regulatory complex; IC, intermediate chain; LC, light chain.

© 2008 Bui et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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