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Published online December 15, 2008
doi:10.1083/jcb.200801157
The Journal of Cell Biology, Vol. 183, No. 6, 1019-1031
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Zhang et al.
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Article

A handcuff model for the cohesin complex



Nenggang Zhang1, Sergey G. Kuznetsov2, Shyam K. Sharan2, Kaiyi Li1, Pulivarthi H. Rao1, and Debananda Pati1

1 Department of Pediatric Hematology/Oncology, Texas Children's Cancer Center, Baylor College of Medicine, Houston, TX 77030
2 Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702

Correspondence to Debananda Pati: pati{at}bcm.tmc.edu

The cohesin complex is responsible for the accurate separation of sister chromatids into two daughter cells. Several models for the cohesin complex have been proposed, but the one-ring embrace model currently predominates the field. However, the static configuration of the embrace model is not flexible enough for cohesins to perform their functions during DNA replication, transcription, and DNA repair. We used coimmunoprecipitation, a protein fragment complement assay, and a yeast two-hybrid assay to analyze the protein–protein interactions among cohesin subunits. The results show that three of the four human cohesin core subunits (Smc1, Smc3, and Rad21) interact with themselves in an Scc3 (SA1/SA2)-dependent manner. These data support a two-ring handcuff model for the cohesin complex, which is flexible enough to establish and maintain sister chromatid cohesion as well as ensure the fidelity of chromosome segregation in higher eukaryotes.

Abbreviations used in this paper: CT, carboxyl terminus; CT-L, CT long; FRET, fluorescence resonance energy transfer; IP, immunoprecipitation; NT, amino terminus; NT-L, NT long; PCA, protein complement assay; WB, Western blot.

© 2008 Zhang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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