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Protein kinase CK2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G

¹ Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 641, Marseille F-13916, France
² Université de la Méditerranée, Faculté de Médecine Secteur-Nord, Institut Fédératif de Recherche 11, Marseille F-13916, France
³ Université de la Méditerranée, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6231, Marseille F-13916, France
⁴ Centre d'Analyse Protéomique de Marseille, Marseille F-13916, France

Correspondence to Bénédicte Dargent: benedicte.dargent{at}univmed.fr

In neurons, generation and propagation of action potentials requires the precise accumulation of sodium channels at the axonal initial segment (AIS) and in the nodes of Ranvier through ankyrin G scaffolding. We found that the ankyrin-binding motif of Na_v1.2 that determines channel concentration at the AIS depends on a glutamate residue (E1111), but also on several serine residues (S1112, S1124, and S1126). We showed that phosphorylation of these residues by protein kinase CK2 (CK2) regulates Na_v channel interaction with ankyrins. Furthermore, we observed that CK2 is highly enriched at the AIS and the nodes of Ranvier in vivo. An ion channel chimera containing the Na_v1.2 ankyrin-binding motif perturbed endogenous sodium channel accumulation at the AIS, whereas phosphorylation-deficient chimeras did not. Finally, inhibition of CK2 activity reduced sodium channel accumulation at the AIS of neurons. In conclusion, CK2 contributes to sodium channel organization by regulating their interaction with ankyrin G.

Abbreviations used in this paper: AIS, axonal initial segment; CK2, protein kinase CK2; CNS, central nervous system; DIV, days in vitro; DMAT, 2-dimethylamino-4,5,6,7-tetrabromo1H-benzimidazole; MAP2, microtubule-associated protein 2; MBD, membrane-binding domain; MBD-ank, MBD-ankyrin; PNS, peripheral nervous system; SPR, surface plasmon resonance.

© 2008 Bréchet et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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