JCB logo
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1083/jcb.200807020
The Journal of Cell Biology, Vol. 183, No. 6, 1129-1143
The Rockefeller University Press, 0021-9525 $30.00
© Harris et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 14210K)
Right arrow PDF+supp data (16647K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harris, K. P.
Right arrow Articles by Tepass, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harris, K. P.
Right arrow Articles by Tepass, U.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Article

Cdc42 and Par proteins stabilize dynamic adherens junctions in the Drosophila neuroectoderm through regulation of apical endocytosis



Kathryn P. Harris and Ulrich Tepass

Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario M5S 3G5, Canada

Correspondence to Ulrich Tepass: u.tepass{at}utoronto.ca

Cell rearrangements require dynamic changes in cell–cell contacts to maintain tissue integrity. We investigated the function of Cdc42 in maintaining adherens junctions (AJs) and apical polarity in the Drosophila melanogaster neuroectodermal epithelium. About one third of cells exit the epithelium through ingression and become neuroblasts. Cdc42-compromised embryos lost AJs in the neuroectoderm during neuroblast ingression. In contrast, when neuroblast formation was suppressed, AJs were maintained despite the loss of Cdc42 function. Loss of Cdc42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs, which are required for AJ stability. In addition, Cdc42 has a second function in regulating endocytotic trafficking, as it is required for the progression of apical cargo from the early to the late endosome. The Par complex acts as an effector for Cdc42 in controlling the endocytosis of apical proteins. This study reveals functional interactions between apical polarity proteins and endocytosis that are critical for stabilizing dynamic basolateral AJs.

Abbreviations used in this paper: AJ, adherens junction; aPKC, atypical PKC; Arm, Armadillo; Avl, Avalanche; Baz, Bazooka; CA, constitutively active; Crb, Crumbs; DEcad, Drosophila epithelial cadherin; DN, dominant negative; Ed, Echinoid; GEF, guanine nucleotide exchange factor; Lva, Lava lamp; Nrt, Neurotactin; Scrib, Scribble; shg, shotgun; UAS, upstream activation sequence; Wasp, Wiskott-Aldrich syndrome protein; ZA, zonula adherens.

© 2008 Harris and Tepass This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

Regulating polarity by directing traffic: Cdc42 prevents adherens junctions from Crumblin' aPart
Mara C. Duncan and Mark Peifer
J. Cell Biol. 2008 183: 971-974. [Abstract] [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents