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Published online
doi:10.1083/jcb.200806151
The Journal of Cell Biology, Vol. 183, No. 6, 979-988
The Rockefeller University Press, 0021-9525 $30.00
© Huang et al.
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Assembly of normal actomyosin rings in the absence of Mid1p and cortical nodes in fission yeast



Yinyi Huang1,2, Hongyan Yan1,2, and Mohan K. Balasubramanian1,2

1 Temasek Life Sciences Laboratory and 2 Department of Biological Sciences, National University of Singapore, Singapore 117604

Correspondence to Mohan K. Balasubramanian: mohan{at}tll.org.sg

Cytokinesis in many eukaryotes depends on the function of an actomyosin contractile ring. The mechanisms regulating assembly and positioning of this ring are not fully understood. The fission yeast Schizosaccharomyces pombe divides using an actomyosin ring and is an attractive organism for the study of cytokinesis. Recent studies in S. pombe (Wu, J.Q., V. Sirotkin, D.R. Kovar, M. Lord, C.C. Beltzner, J.R. Kuhn, and T.D. Pollard. 2006. J. Cell Biol. 174:391–402; Vavylonis, D., J.Q. Wu, S. Hao, B. O'Shaughnessy, and T.D. Pollard. 2008. Science. 319:97–100) have suggested that the assembly of the actomyosin ring is initiated from a series of cortical nodes containing several components of this ring. These studies have proposed that actomyosin interactions bring together the cortical nodes to form a compacted ring structure. In this study, we test this model in cells that are unable to assemble cortical nodes. Although the cortical nodes play a role in the timing of ring assembly, we find that they are dispensable for the assembly of orthogonal actomyosin rings. Thus, a mechanism that is independent of cortical nodes is sufficient for the assembly of normal actomyosin rings.

Abbreviations used in this paper: F-actin, filamentous actin; HU, hydroxyurea; LatA, latrunculin A; SIN, septation initiation network; SPB, spindle pole body.

© 2008 Huang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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