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–E-catenin binds to dynamitin and regulates dynactin-mediated intracellular traffic
Correspondence to Valeri Vasioukhin: vvasiouk{at}fhcrc.org
–Epithelial catenin (E-catenin) is an important cell–cell adhesion protein. In this study, we show that –E-catenin also regulates intracellular traffic by binding to the dynactin complex component dynamitin. Dynactin-mediated organelle trafficking is increased in –E-catenin–/– keratinocytes, an effect that is reversed by expression of exogenous –E-catenin. Disruption of adherens junctions in low-calcium media does not affect dynactin-mediated traffic, indicating that –E-catenin regulates traffic independently from its function in cell–cell adhesion. Although neither the integrity of dynactin–dynein complexes nor their association with vesicles is affected by –E-catenin, –E-catenin is necessary for the attenuation of microtubule-dependent trafficking by the actin cytoskeleton. Because the actin-binding domain of –E-catenin is necessary for this regulation, we hypothesize that –E-catenin functions as a dynamic link between the dynactin complex and actin and, thus, integrates the microtubule and actin cytoskeleton during intracellular trafficking.
© 2008 Lien et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
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