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Published online December 29, 2008
doi:10.1083/jcb.200806048
The Journal of Cell Biology, Vol. 183, No. 7, 1213-1221
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Kutik et al.
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The translocator maintenance protein Tam41 is required for mitochondrial cardiolipin biosynthesis



Stephan Kutik1,2,3, Michael Rissler1, Xue Li Guan4,5, Bernard Guiard6, Guanghou Shui4,5, Natalia Gebert1,2,3, Philip N. Heacock7,8, Peter Rehling9, William Dowhan7,8, Markus R. Wenk4,5, Nikolaus Pfanner1,2, and Nils Wiedemann1

1 Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, 2 Center for Biological Signalling Studies, and 3 Fakultät für Biologie, Universität Freiburg, 79104 Freiburg, Germany
4 Department of Biochemistry and 5 Department of Biological Sciences, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119077, Singapore
6 Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France
7 Department of Biochemistry and Molecular Biology and 8 Center for Membrane Biology, University of Texas-Houston Medical School, Houston, TX 77225
9 Abteilung für Biochemie II, Universität Göttingen, 37073 Göttingen, Germany

Correspondence to Nikolaus Pfanner: nikolaus.pfanner{at}biochemie.uni-freiburg.de

The mitochondrial inner membrane contains different translocator systems for the import of presequence-carrying proteins and carrier proteins. The translocator assembly and maintenance protein 41 (Tam41/mitochondrial matrix protein 37) was identified as a new member of the mitochondrial protein translocator systems by its role in maintaining the integrity and activity of the presequence translocase of the inner membrane (TIM23 complex). Here we demonstrate that the assembly of proteins imported by the carrier translocase, TIM22 complex, is even more strongly affected by the lack of Tam41. Moreover, respiratory chain supercomplexes and the inner membrane potential are impaired by lack of Tam41. The phenotype of Tam41-deficient mitochondria thus resembles that of mitochondria lacking cardiolipin. Indeed, we found that Tam41 is required for the biosynthesis of the dimeric phospholipid cardiolipin. The pleiotropic effects of the translocator maintenance protein on preprotein import and respiratory chain can be attributed to its role in biosynthesis of mitochondrial cardiolipin.

S. Kutik and M. Rissler contributed equally to this paper.

Abbreviations used in this paper: AAC, ADP/ATP carrier; Crd1, cardiolipin synthase; DIC, dicarboxylate carrier; LCMS, liquid chromatography/mass spectrometry; Mmp37, mitochondrial matrix protein 37; PA, phosphatidic acid; PAM, presequence translocase-associated motor; PG, phosphatidylglycerol; PGP, phosphatidylglycerophosphate; Pgs1, PGP synthase; Tam41, translocator assembly and maintenance protein 41; TIM, translocase of the inner membrane.

© 2008 Kutik et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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