Published online December 22, 2008
doi:10.1083/jcb.200809190
The Journal of Cell Biology, Vol. 183, No. 7, 1223-1233
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Bieling et al.
CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites
Peter Bieling1,
Stefanie Kandels-Lewis1,
Ivo A. Telley1,
Juliette van Dijk2,
Carsten Janke2, and
Thomas Surrey1
1 European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, 69117 Heidelberg, Germany
2 Centre de Recherche de Biochimie Macromoléculaire, Université Montpellier 2 and 1, Centre National de la Recherche Scientifique, 34293 Montpellier, France
Correspondence to Thomas Surrey: surrey{at}embl.de
The microtubule cytoskeleton is crucial for the internal organization of eukaryotic cells. Several microtubule-associated proteins link microtubules to subcellular structures. A subclass of these proteins, the plus end–binding proteins (+TIPs), selectively binds to the growing plus ends of microtubules. Here, we reconstitute a vertebrate plus end tracking system composed of the most prominent +TIPs, end-binding protein 1 (EB1) and CLIP-170, in vitro and dissect their end-tracking mechanism. We find that EB1 autonomously recognizes specific binding sites present at growing microtubule ends. In contrast, CLIP-170 does not end-track by itself but requires EB1. CLIP-170 recognizes and turns over rapidly on composite binding sites constituted by end-accumulated EB1 and tyrosinated 
-tubulin. In contrast to its fission yeast orthologue Tip1, dynamic end tracking of CLIP-170 does not require the activity of a molecular motor. Our results demonstrate evolutionary diversity of the plus end recognition mechanism of CLIP-170 family members, whereas the autonomous end-tracking mechanism of EB family members is conserved.
Abbreviations used in this paper: CAP-gly, cytoskeleton-associated protein glycine-rich; EB, end-binding protein; PEG, polyethylene glycol; PLL, poly-L-lysine; TAMRA, tetramethyl-6-carboxyrhodamine; TIRF, total internal reflection fluorescence.
© 2008 Bieling et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
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