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Published online
doi:10.1083/jcb.200806062
The Journal of Cell Biology, Vol. 183, No. 7, 1275-1286
The Rockefeller University Press, 0021-9525 $30.00
© Matsuzaki et al.
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Article

The peroxisomal membrane protein import receptor Pex3p is directly transported to peroxisomes by a novel Pex19p- and Pex16p-dependent pathway



Takashi Matsuzaki1 and Yukio Fujiki1,2

1 Department of Biology, Faculty of Sciences, Kyushu University Graduate School, Fukuoka 812-8581, Japan
2 Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Chiyoda, Tokyo 102-0075, Japan

Correspondence to Yukio Fujiki: yfujiscb{at}mbox.nc.kyushu-u.ac.jp

Two distinct pathways have recently been proposed for the import of peroxisomal membrane proteins (PMPs): a Pex19p- and Pex3p-dependent class I pathway and a Pex19p- and Pex3p-independent class II pathway. We show here that Pex19p plays an essential role as the chaperone for full-length Pex3p in the cytosol. Pex19p forms a soluble complex with newly synthesized Pex3p in the cytosol and directly translocates it to peroxisomes. Knockdown of Pex19p inhibits peroxisomal targeting of newly synthesized full-length Pex3p and results in failure of the peroxisomal localization of Pex3p. Moreover, we demonstrate that Pex16p functions as the Pex3p-docking site and serves as the peroxisomal membrane receptor that is specific to the Pex3p–Pex19p complexes. Based on these novel findings, we suggest a model for the import of PMPs that provides new insights into the molecular mechanisms underlying the biogenesis of peroxisomes and its regulation involving Pex3p, Pex19p, and Pex16p.

Abbreviations used in this paper: HA, influenza virus hemagglutinin; mPTS, peroxisomal membrane-targeting signal; PMP, peroxisomal membrane protein; PNS, postnuclear supernatant; SB, semi-intact cell buffer; TM, transmembrane.

© 2008 Matsuzaki and Fujiki This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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