JCB logo
Quantitative Colocalization Analysis Software
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online December 29, 2008
doi:10.1083/jcb.200807023
The Journal of Cell Biology, Vol. 183, No. 7, 1287-1298
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Lewkowicz et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1886K)
Right arrow PDF+supp data (3448K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lewkowicz, E.
Right arrow Articles by Niedergang, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lewkowicz, E.
Right arrow Articles by Niedergang, F.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

The microtubule-binding protein CLIP-170 coordinates mDia1 and actin reorganization during CR3-mediated phagocytosis



Elodie Lewkowicz1,2, Floriane Herit1,2, Christophe Le Clainche3, Pierre Bourdoncle1,2, Franck Perez4,5, and Florence Niedergang1,2

1 Institut Cochin, Université Paris Descartes, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8104, 75014 Paris, France
2 Institut national de la santé et de la recherche médicale, Unité 567, 75014 Paris, France
3 Centre National de la Recherche Scientifique, Unité Propre de Recherche 3082, 91190 Gif-sur-Yvette, France
4 Institut Curie, Centre de Recherche, 75248 Paris, France
5 Centre National de la Recherche Scientifique, Unité Mixte de Recherche 144, 75248 Paris, France

Correspondence to Florence Niedergang: florence.niedergang{at}inserm.fr

Microtubule dynamics are modulated by regulatory proteins that bind to their plus ends (+TIPs [plus end tracking proteins]), such as cytoplasmic linker protein 170 (CLIP-170) or end-binding protein 1 (EB1). We investigated the role of +TIPs during phagocytosis in macrophages. Using RNA interference and dominant-negative approaches, we show that CLIP-170 is specifically required for efficient phagocytosis triggered by {alpha}Mβ2 integrin/complement receptor activation. This property is not observed for EB1 and EB3. Accordingly, whereas CLIP-170 is dynamically enriched at the site of phagocytosis, EB1 is not. Furthermore, we observe that CLIP-170 controls the recruitment of the formin mDia1, an actin-nucleating protein, at the onset of phagocytosis and thereby controls actin polymerization events that are essential for phagocytosis. CLIP-170 directly interacts with the formin homology 2 domain of mDia1. The interaction between CLIP-170 and mDia1 is negatively regulated during {alpha}Mβ2-mediated phagocytosis. Our results unravel a new microtubule/actin cooperation that involves CLIP-170 and mDia1 and that functions downstream of {alpha}Mβ2 integrins.

Abbreviations used in this paper: CLIP, cytoplasmic linker protein; CR3, complement receptor 3; DAD, diaphanous autoregulatory domain; EB, end-binding protein; FcR, Fc receptor; FH2, formin homology 2; shRNA, small hairpin RNA; SRBC, sheep red blood cell.

© 2008 Lewkowicz et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents