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Published online January 12, 2009
doi:10.1083/jcb.200806044
The Journal of Cell Biology, Vol. 184, No. 1, 113-127
The Rockefeller University Press, 0021-9525 $30.00
© 2009 Roberts-Galbraith et al.
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Article

The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring



Rachel H. Roberts-Galbraith1,2, Jun-Song Chen1,2, Jianqiu Wang1,2, and Kathleen L. Gould1,2

1 Howard Hughes Medical Institute, Chevy Chase, MD 20815
2 Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232

Correspondence to Kathleen L. Gould: kathy.gould{at}vanderbilt.edu

Schizosaccharomyces pombe cdc15 homology (PCH) family members participate in many cellular processes by bridging the plasma membrane and cytoskeleton. Their F-BAR domains bind and curve membranes, whereas other domains, typically SH3 domains, are expected to provide cytoskeletal links. We tested this prevailing model of functional division in the founding member of the family, Cdc15, which is essential for cytokinesis in S. pombe, and in the related PCH protein, Imp2. We find that the distinct functions of Imp2 and Cdc15 are SH3 domain independent. However, the Cdc15 and Imp2 SH3 domains share an essential role in recruiting proteins to the contractile ring, including Pxl1 and Fic1. Together, Pxl1 and Fic1, a previously uncharacterized C2 domain protein, add structural integrity to the contractile ring and prevent it from fragmenting during division. Our data indicate that the F-BAR proteins Cdc15 and Imp2 contribute to a single biological process with both distinct and overlapping functions.

Abbreviations used in this paper: MBP, maltose-binding protein; MS, mass spectrometry; PCH, S. pombe cdc15 homology; TAP, tandem affinity purification; TEV, tobacco etch virus; YE, yeast extract.

© 2009 Roberts-Galbraith et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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