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Published online
doi:10.1083/jcb.200808068
The Journal of Cell Biology, Vol. 184, No. 1, 129-141
The Rockefeller University Press, 0021-9525 $30.00
© Tamura et al.
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Article

Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import



Yasushi Tamura1, Yoshihiro Harada1, Takuya Shiota1, Koji Yamano1, Kazuaki Watanabe1, Mihoko Yokota1, Hayashi Yamamoto1, Hiromi Sesaki2, and Toshiya Endo1

1 Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan
2 Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205

Correspondence to Toshiya Endo: endo{at}biochem.chem.nagoya-u.ac.jp

Mitochondrial protein traffic requires coordinated operation of protein translocator complexes in the mitochondrial membrane. The TIM23 complex translocates and inserts proteins into the mitochondrial inner membrane. Here we analyze the intermembrane space (IMS) domains of Tim23 and Tim50, which are essential subunits of the TIM23 complex, in these functions. We find that interactions of Tim23 and Tim50 in the IMS facilitate transfer of precursor proteins from the TOM40 complex, a general protein translocator in the outer membrane, to the TIM23 complex. Tim23–Tim50 interactions also facilitate a late step of protein translocation across the inner membrane by promoting motor functions of mitochondrial Hsp70 in the matrix. Therefore, the Tim23–Tim50 pair coordinates the actions of the TOM40 and TIM23 complexes together with motor proteins for mitochondrial protein import.

Y. Tamura's present address is Dept. of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205.

H. Yamamoto's present address is Dept. of Cell Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan.

Abbreviations used in this paper: BPA, benzoylphenylalanine; DHFR, mouse dihydrofolate reductase; IMS, intermembrane space; MMC, mtHsp70-associated motor and chaperone; mtHsp70, mitochondrial Hsp70; PK, proteinase K.

© 2009 Tamura et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

The Rockefeller University Press


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