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Published online
doi:10.1083/jcb.200810041
The Journal of Cell Biology, Vol. 184, No. 4, 481-490
The Rockefeller University Press, 0021-9525 $30.00
© Doyle et al.
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One-dimensional topography underlies three-dimensional fibrillar cell migration



Andrew D. Doyle1, Francis W. Wang2, Kazue Matsumoto1, and Kenneth M. Yamada1

1 Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892
2 Polymers Division, Material Science and Engineering Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899

Correspondence to Andrew D. Doyle: adoyle{at}mail.nih.gov; or Kenneth M. Yamada: kyamada{at}mail.nih.gov

Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (µPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and dependent on myosin II contractility and microtubules (MTs). 1D and 3D migration are also characterized by an anterior MT bundle with a posterior centrosome. We propose that cells migrate rapidly through 3D fibrillar matrices by a 1D migratory mechanism not mimicked by 2D matrices.

Abbreviations used in this paper: FN, fibronectin; HK, human keratinocyte; MT, microtubule; µPP, microphotopatterning; PVA, polyvinyl alcohol; ROI, region of interest; TIRF, total internal reflection fluorescence.


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