JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1083/jcb.200806019
The Journal of Cell Biology, Vol. 185, No. 1, 163-176
The Rockefeller University Press, 0021-9525 $30.00
© Schneider et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 4718K)
Right arrow PDF+supp data (6223K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneider, L.
Right arrow Articles by Pedersen, S. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneider, L.
Right arrow Articles by Pedersen, S. F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Article

The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-{alpha} in the primary cilium



Linda Schneider1, Christian-Martin Stock2, Peter Dieterich3, Bo Hammer Jensen1, Lotte Bang Pedersen1, Peter Satir4, Albrecht Schwab2, Søren Tvorup Christensen1, and Stine Falsig Pedersen1

1 Department of Biology, University of Copenhagen, DK-2100 Copenhagen O, Denmark
2 Institut für Physiologie, Universität Münster, D-48149 Münster, Germany
3 Institut für Physiologie, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
4 Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461

Correspondence to Stine Falsig Pedersen: sfpedersen{at}bio.ku.dk

We previously demonstrated that the primary cilium coordinates platelet-derived growth factor (PDGF) receptor (PDGFR) {alpha}–mediated migration in growth-arrested fibroblasts. In this study, we investigate the functional relationship between ciliary PDGFR-{alpha} and the Na+/H+ exchanger NHE1 in directional cell migration. NHE1 messenger RNA and protein levels are up-regulated in NIH3T3 cells and mouse embryonic fibroblasts (MEFs) during growth arrest, which is concomitant with cilium formation. NHE1 up-regulation is unaffected in Tg737orpk MEFs, which have no or very short primary cilia. In growth-arrested NIH3T3 cells, NHE1 is activated by the specific PDGFR-{alpha} ligand PDGF-AA. In wound-healing assays on growth-arrested NIH3T3 cells and wild-type MEFs, NHE1 inhibition by 5'-(N-ethyl-N-isopropyl) amiloride potently reduces PDGF-AA–mediated directional migration. These effects are strongly attenuated in interphase NIH3T3 cells, which are devoid of primary cilia, and in Tg737orpk MEFs. PDGF-AA failed to stimulate migration in NHE1-null fibroblasts. In conclusion, stimulation of directional migration in response to ciliary PDGFR-{alpha} signals is specifically dependent on NHE1 activity, indicating that NHE1 activation is a critical event in the physiological response to PDGFR-{alpha} stimulation.

© 2009 Schneider et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

This work was presented at the American Society for Cell Biology meeting in Washington, DC, on 5 December 2007 and has appeared in abstract form.

Abbreviations used in this paper: ANOVA, analysis of variance; EIPA, 5'-(N-ethyl-N-isopropyl) amiloride; gas, growth arrest specific; MEF, mouse embryonic fibroblast; PDGFR, PDGF receptor; pHi, intracellular pH; WT, wild type.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents