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Published online
doi:10.1083/jcb.200807155
The Journal of Cell Biology, Vol. 185, No. 2, 341-355
The Rockefeller University Press, 0021-9525 $30.00
© Fejtova et al.
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Article

Dynein light chain regulates axonal trafficking and synaptic levels of Bassoon



Anna Fejtova1, Daria Davydova1, Ferdinand Bischof1, Vesna Lazarevic1, Wilko D. Altrock1, Stefano Romorini1, Cornelia Schöne1, Werner Zuschratter2, Michael R. Kreutz3, Craig C. Garner4, Noam E. Ziv5,6,7, and Eckart D. Gundelfinger1

1 Department of Neurochemistry and Molecular Biology, 2 Special Laboratory for Electron and Laserscannning Microscopy, and 3 Project Group Neuroplasticity, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany
4 Nancy Pritzker Laboratory, Department of Psychiatry and Behavioral Science, Stanford University School of Medicine, Stanford, CA 94305
5 The Rappaport Family Institute for Research in Medical Sciences, 6 Department of Physiology and Biophysics, and 7 The Lorry Lokey Interdisciplinary Center for Life Sciences and Engineering, Technion–Israel Institute of Technology, Haifa 32000, Israel

Correspondence to Anna Fejtova: afejtova{at}ifn-magdeburg.de; or Eckart D. Gundelfinger: gundelfi{at}ifn-magdeburg.de

Bassoon and the related protein Piccolo are core components of the presynaptic cytomatrix at the active zone of neurotransmitter release. They are transported on Golgi-derived membranous organelles, called Piccolo-Bassoon transport vesicles (PTVs), from the neuronal soma to distal axonal locations, where they participate in assembling new synapses. Despite their net anterograde transport, PTVs move in both directions within the axon. How PTVs are linked to retrograde motors and the functional significance of their bidirectional transport are unclear. In this study, we report the direct interaction of Bassoon with dynein light chains (DLCs) DLC1 and DLC2, which potentially link PTVs to dynein and myosin V motor complexes. We demonstrate that Bassoon functions as a cargo adapter for retrograde transport and that disruption of the Bassoon–DLC interactions leads to impaired trafficking of Bassoon in neurons and affects the distribution of Bassoon and Piccolo among synapses. These findings reveal a novel function for Bassoon in trafficking and synaptic delivery of active zone material.

Abbreviations used in this paper: DBM, DLC-binding mutant; DHC, dynein heavy chain; DIV, day in vitro; DLC, dynein light chain; ICC, immunocytochemistry; mRFP, monomeric RFP, MTOC, microtubule-organizing center; OC, overlap coefficient; PTV, Piccolo-Bassoon transport vesicle; SV, synaptic vesicle; WB, Western blot.

© 2009 Fejtova et al.
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