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Formation of cristae and crista junctions in mitochondria depends on antagonism between Fcj1 and Su e/g

¹ Adolf-Butenandt Institute for Physiological Chemistry, and ² Center for Integrated Protein Science Munich, Ludwig-Maximilians University, 81377 Munich, Germany
³ University of Ottawa Heart Institute, Ottawa, Ontario K1Y 4W7, Canada
⁴ Max-Planck-Institut für Biochemie, 82152 Martinsried, Germany
⁵ Max-Delbrück-Centrum für molekulare Medizin Berlin-Buch, 13092 Berlin, Germany
⁶ Institute of Developmental Genetics, Hemholtz Center Munich, German Research Center for Environmental Health, GmbH, 85764 Neuherberg, Germany
⁷ Mitochondrial Biology, Cluster of Excellence Frankfurt Macromolecular Complexes, Johann Wolfgang Goethe University Medical School, 60590 Frankfurt am Main, Germany

Correspondence to Andreas S. Reichert: reichert{at}zbc.kgu.de

Crista junctions (CJs) are important for mitochondrial organization and function, but the molecular basis of their formation and architecture is obscure. We have identified and characterized a mitochondrial membrane protein in yeast, Fcj1 (formation of CJ protein 1), which is specifically enriched in CJs. Cells lacking Fcj1 lack CJs, exhibit concentric stacks of inner membrane in the mitochondrial matrix, and show increased levels of F₁F_O–ATP synthase (F₁F_O) supercomplexes. Overexpression of Fcj1 leads to increased CJ formation, branching of cristae, enlargement of CJ diameter, and reduced levels of F₁F_O supercomplexes. Impairment of F₁F_O oligomer formation by deletion of its subunits e/g (Su e/g) causes CJ diameter enlargement and reduction of cristae tip numbers and promotes cristae branching. Fcj1 and Su e/g genetically interact. We propose a model in which the antagonism between Fcj1 and Su e/g locally modulates the F₁F_O oligomeric state, thereby controlling membrane curvature of cristae to generate CJs and cristae tips.

Abbreviations used in this paper: BN-PAGE, blue native PAGE; CJ, crista junction; CM, cristae membrane; DIC, differential interference contrast; IBM, inner boundary membrane; IM, inner membrane; mtDNA, mitochondrial DNA; OM, outer membrane; PK, proteinase K; SEC, size exclusion chromatography; TAP, tandem affinity purification.

© 2009 Rabl et al.
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