JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1083/jcb.200812025
The Journal of Cell Biology, Vol. 185, No. 7, 1195-1207
The Rockefeller University Press, 0021-9525 $30.00
© Saint-Marcoux et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 5100K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Saint-Marcoux, D.
Right arrow Articles by de Vitry, C.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saint-Marcoux, D.
Right arrow Articles by de Vitry, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Article

Biogenesis of cytochrome b6 in photosynthetic membranes



Denis Saint-Marcoux1,2, Francis-André Wollman1,2, and Catherine de Vitry1,2

1 Centre National de la Recherche Scientifique, UMR 7141, Physiologie Membranaire et Moléculaire du Chloroplaste, Institut de Biologie Physico-Chimique, 75005 Paris, France
2 Université Pierre et Marie Curie, Paris Universitas 06, UMR 7141, 75005 Paris, France

Correspondence to Catherine de Vitry: catherine.devitry{at}ibpc.fr

In chloroplasts, binding of a c'-heme to cytochrome b6 on the stromal side of the thylakoid membranes requires a specific mechanism distinct from the one at work for c-heme binding to cytochromes f and c6 on the lumenal side of membranes. Here, we show that the major protein components of this pathway, the CCBs, are bona fide transmembrane proteins. We demonstrate their association in a series of hetero-oligomeric complexes, some of which interact transiently with cytochrome b6 in the process of heme delivery to the apoprotein. In addition, we provide preliminary evidence for functional assembly of cytochrome b6f complexes even in the absence of c'-heme binding to cytochrome b6. Finally, we present a sequential model for apo- to holo-cytochrome b6 maturation integrated within the assembly pathway of b6f complexes in the thylakoid membranes.

Abbreviations used in this paper: BN, blue native; CCB, cofactor assembly on complex C subunit B; Ccm, cytochrome c maturation; CCS, cytochrome c synthesis; cyt, cytochrome; n side, negative side; OEE, oxygen evolution enhancer; PS, photosystem; p side, positive side; TMD, transmembrane domain; WT, wild type.

© 2009 Saint-Marcoux et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents