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Published online
doi:10.1083/jcb.200903100
The Journal of Cell Biology, Vol. 186, No. 2, 173-182
The Rockefeller University Press, 0021-9525 $30.00
© Amano et al.
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The CENP-S complex is essential for the stable assembly of outer kinetochore structure



Miho Amano1, Aussie Suzuki1, Tetsuya Hori1, Chelsea Backer2,3, Katsuya Okawa4, Iain M. Cheeseman2,3, and Tatsuo Fukagawa1

1 Department of Molecular Genetics, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan
2 Whitehead Institute for Biomedical Research and 3 Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142
4 Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan

Correspondence to Tatsuo Fukagawa: tfukagaw{at}lab.nig.ac.jp

The constitutive centromere-associated network (CCAN) proteins are central to kinetochore assembly. To define the molecular architecture of this critical kinetochore network, we sought to determine the full complement of CCAN components and to define their relationships. This work identified a centromere protein S (CENP-S)–containing subcomplex that includes the new constitutive kinetochore protein CENP-X. Both CENP-S– and CENP-X–deficient chicken DT40 cells are viable but show abnormal mitotic behavior based on live cell analysis. Human HeLa cells depleted for CENP-X also showed mitotic errors. The kinetochore localization of CENP-S and -X is abolished in CENP-T– or CENP-K–deficient cells, but reciprocal experiments using CENP-S–deficient cells did not reveal defects in the localization of CCAN components. However, CENP-S– and CENP-X–deficient cells show a significant reduction in the size of the kinetochore outer plate. In addition, we found that intrakinetochore distance was increased in CENP-S– and CENP-X–deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore.

M. Amano, A. Suzuki, and T. Hori contributed equally to this paper.

Abbreviations used in this paper: CCAN, constitutive centromere-associated network; IP, immunoprecipitation; KMN, KNL1–Mis12 complex–Ndc80 complex; KO, knockout

© 2009 Amano et al.
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