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Published online
doi:10.1083/jcb.200810147
The Journal of Cell Biology, Vol. 186, No. 4, 555-570
The Rockefeller University Press, 0021-9525 $30.00
© Jung et al.
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Article

The Dictyostelium type V myosin MyoJ is responsible for the cortical association and motility of contractile vacuole membranes



Goeh Jung1, Margaret A. Titus2, and John A. Hammer, III1

1 Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
2 Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455

Correspondence to John A. Hammer III: hammerj{at}nhlbi.nih.gov

The contractile vacuole (CV) complex in Dictyostelium is a tubulovesicular osmoregulatory organelle that exhibits extensive motility along the actin-rich cortex, providing a useful model for investigating myosin-dependent membrane transport. Here, we show that the type V myosin myoJ localizes to CV membranes and is required for efficient osmoregulation, the normal accumulation of CV membranes in the cortex, and the conversion of collapsed bladder membranes into outwardly radiating cortical CV tubules. Complementation of myoJ-null cells with a version of myoJ containing a shorter lever arm causes these radiating tubules to move at a slower speed, confirming myoJ's role in translocating CV membranes along the cortex. MyoJ-null cells also exhibit a dramatic concentration of CV membranes around the microtubule-organizing center. Consistently, we demonstrate that CV membranes also move bi-directionally on microtubules between the cortex and the centrosome. Therefore, myoJ cooperates with plus and minus end–directed microtubule motors to drive the normal distribution and dynamics of the CV complex in Dictyostelium.

Abbreviations used in this paper: CV, contractile vacuole; GTD, globular tail domain; MTOC, microtubule-organizing center; NZ, nocodazole; WT, wild type.

© 2009 The Rockefeller University Press
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