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Published online
doi:10.1083/jcb.200906098
The Journal of Cell Biology, Vol. 186, No. 6, 793-803
The Rockefeller University Press, 0021-9525 $30.00
© DeVay et al.
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Coassembly of Mgm1 isoforms requires cardiolipin and mediates mitochondrial inner membrane fusion



Rachel M. DeVay, Lenin Dominguez-Ramirez, Laura L. Lackner, Suzanne Hoppins, Henning Stahlberg, and Jodi Nunnari

Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616

Correspondence to Jodi Nunnari: jmnunnari{at}ucdavis.edu

Two dynamin-related protein (DRP) families are essential for fusion of the outer and inner mitochondrial membranes, Fzo1 (yeast)/Mfn1/Mfn2 (mammals) and Mgm1 (yeast)/Opa1 (mammals), respectively. Fzo1/Mfns possess two medial transmembrane domains, which place their critical GTPase and coiled-coil domains in the cytosol. In contrast, Mgm1/Opa1 are present in cells as long (l) isoforms that are anchored via the N terminus to the inner membrane, and short (s) isoforms were predicted to be soluble in the intermembrane space. We addressed the roles of Mgm1 isoforms and how DRPs function in membrane fusion. Our analysis indicates that in the absence of a membrane, l- and s-Mgm1 both exist as inactive GTPase monomers, but that together in trans they form a functional dimer in a cardiolipin-dependent manner that is the building block for higher-order assemblies.

Abbreviations used in this paper: CCD, charge-coupled device; CL, cardiolipin; DRP, dynamin-related protein; IMC, inner mitochondrial membrane composition; OMC, outer membrane composition.

© 2009 DeVay et al.
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