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The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice

¹ Department of Cell Biology, School of Medicine and ² Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205
³ Department of Anatomy, Hokkaido University, Sapporo 060-8638, Japan

Correspondence to Hiromi Sesaki: hsesaki{at}jhmi.edu

The dynamin-related guanosine triphosphatase Drp1 mediates the division of mitochondria and peroxisomes. To understand the in vivo function of Drp1, complete and tissue-specific mouse knockouts of Drp1 were generated. Drp1-null mice die by embryonic day 11.5. This embryonic lethality is not likely caused by gross energy deprivation, as Drp1-null cells showed normal intracellular adenosine triphosphate levels. In support of the role of Drp1 in organelle division, mitochondria formed extensive networks, and peroxisomes were elongated in Drp1-null embryonic fibroblasts. Brain-specific Drp1 ablation caused developmental defects of the cerebellum in which Purkinje cells contained few giant mitochondria instead of the many short tubular mitochondria observed in control cells. In addition, Drp1-null embryos failed to undergo developmentally regulated apoptosis during neural tube formation in vivo. However, Drp1-null embryonic fibroblasts have normal responses to apoptotic stimuli in vitro, suggesting that the apoptotic function of Drp1 depends on physiological cues. These findings clearly demonstrate the physiological importance of Drp1-mediated organelle division in mice.

Abbreviations used in this paper: CCD, charge-coupled device; En1, engrailed 1; ES, embryonic stem; Flp, flipase; MEF, mouse embryonic fibroblast; PC, Purkinje cell.

© 2009 Wakabayashi et al.
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This article has been cited by other articles:

• Wakabayashi, J., Zhang, Z., Wakabayashi, N., Tamura, Y., Fukaya, M., Kensler, T. W., Iijima, M., Sesaki, H. (2009). The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice. JEM 206: i23-i23 [Full Text]  

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