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Published online
doi:10.1083/jcb.200904111
The Journal of Cell Biology, Vol. 186, No. 6, 863-880
The Rockefeller University Press, 0021-9525 $30.00
© Kemmler et al.
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Article

Yvh1 is required for a late maturation step in the 60S biogenesis pathway



Stefan Kemmler1, Laura Occhipinti1,2, Maria Veisu1,2, and Vikram Govind Panse1

1 Institute of Biochemistry, ETH Zürich, CH-8093 Zürich, Switzerland
2 Molecular Life Sciences Program, Life Sciences Zürich, CH-8057 Zürich, Switzerland

Correspondence to Vikram Govind Panse: vikram.panse{at}bc.biol.ethz.ch

Before entering translation, preribosomal particles undergo sequential late maturation steps. In the case of pre-60S particles, these steps involve the release of shuttling maturation factors and transport receptors. In this study, we report a new maturation step in the 60S biogenesis pathway in budding yeast. We show that efficient release of the nucleolar/nuclear ribosomal-like protein Mrt4 (homologous to the acidic ribosomal P-protein Rpp0) from pre-60S particles requires the highly conserved protein Yvh1, which associates only with late pre-60S particles. Cell biological and biochemical analyses reveal that Mrt4 fails to dissociate from late pre-60S particles in yvh1{Delta} cells, inducing a delay in nuclear pre–ribosomal RNA processing and a pre-60S export defect in yvh1{Delta} cells. Moreover, we have isolated gain of function alleles of Mrt4 that specifically bypass the requirement for Yvh1 and rescue all yvh1{Delta}-associated phenotypes. Together, our data suggest that Yvh1-mediated release of Mrt4 precedes cytoplasmic loading of Rpp0 on pre-60S particles and is an obligatory late step toward construction of translation-competent 60S subunits.

Abbreviations used in this paper: LMB, leptomycin B; r-protein, ribosomal protein; rRNA, ribosomal RNA; TAP, tandem affinity purification.

© 2009 Kemmler et al.
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