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Published online
doi:10.1083/jcb.200812084
The Journal of Cell Biology, Vol. 187, No. 1, 81-89
The Rockefeller University Press, 0021-9525 $30.00
© Engel et al.
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Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model



Benjamin D. Engel, William B. Ludington, and Wallace F. Marshall

Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158

Correspondence to Wallace F. Marshall: wallace.marshall{at}biochem.ucsf.edu

The assembly and maintenance of eukaryotic flagella are regulated by intraflagellar transport (IFT), the bidirectional traffic of IFT particles (recently renamed IFT trains) within the flagellum. We previously proposed the balance-point length control model, which predicted that the frequency of train transport should decrease as a function of flagellar length, thus modulating the length-dependent flagellar assembly rate. However, this model was challenged by the differential interference contrast microscopy observation that IFT frequency is length independent. Using total internal reflection fluorescence microscopy to quantify protein traffic during the regeneration of Chlamydomonas reinhardtii flagella, we determined that anterograde IFT trains in short flagella are composed of more kinesin-associated protein and IFT27 proteins than trains in long flagella. This length-dependent remodeling of train size is consistent with the kinetics of flagellar regeneration and supports a revised balance-point model of flagellar length control in which the size of anterograde IFT trains tunes the rate of flagellar assembly.

Abbreviations used in this paper: CCD, charge-coupled device; DIC, differential interference contrast; IFT, intraflagellar transport; KAP, kinesin-associated protein; ROI, region of interest; TAP, Tris-acetate-phosphate; TIRF, total internal reflection fluorescence.

© 2009 Engel et al.
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