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Published online
doi:10.1083/jcb.200902093
The Journal of Cell Biology, Vol. 187, No. 3, 399-412
The Rockefeller University Press, 0021-9525 $30.00
© Courtheoux et al.
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Article

Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast



Thibault Courtheoux1,2, Guillaume Gay1,2, Yannick Gachet1,2, and Sylvie Tournier1,2

1 Université de Toulouse and 2 Centre National de la Recherche Scientifique, Laboratoire de Biologie Cellulaire et Moléculaire du Controle de la Prolifération UMR5088, F-31062 Toulouse, France

Correspondence to Sylvie Tournier: tournier{at}cict.fr; or Yannick Gachet: gachet{at}cict.fr

Faithful segregation of sister chromatids requires the attachment of each kinetochore (Kt) to microtubules (MTs) that extend from opposite spindle poles. Merotelic Kt orientation is a Kt–MT misattachment in which a single Kt binds MTs from both spindle poles rather than just one. Genetic induction of merotelic Kt attachment during anaphase in fission yeast resulted in intra-Kt stretching followed by either correction or Kt disruption. Laser ablation of spindle MTs revealed that intra-Kt stretching and merotelic correction were dependent on MT forces. The presence of multiple merotelic chromosomes linearly antagonized the spindle elongation rate, and this phenomenon could be solved numerically using a simple force balance model. Based on the predictions of our mechanical model, we provide in vivo evidence that correction of merotelic attachment in anaphase is tension dependent and requires an Ase1/Prc1-dependent mechanism that prevents spindle collapse and thus asymmetric division and/or the appearance of the cut phenotype.

Abbreviations used in this paper: Kt, kinetochore; MT, microtubule; SAC, spindle assembly checkpoint; SPB, spindle pole body; wt, wild type.

© 2009 Courtheoux et al.
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