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The Journal of Cell Biology, Vol 21, 105-114, Copyright © 1964 by Rockefeller University Press

ARTICLE

THE FATE OF DNA-CONTAINING PARTICLES PHAGOCYTIZED BY MAMMALIAN CELLS



Klaus Bensch M.D.1, Gerald Gordon M.D.1, and Leonard Miller M.D.1

1 From Yale University School of Medicine, Department of Pathology, New Haven

Particulate DNA protein coacervates were digested immediately after being phagocytized by L strain fibroblasts in suspension culture. Enlargement of the phagocytotic vacuoles occurred simultaneously with a loss of the electron opacity of the phagocytized particles. Cytochemical reactions positive for non-specific esterase, acid phosphatase, and nucleoside phosphatase in the phagocytotic vacuoles provided additional evidence for the probability of complete hydrolysis of the phagocytized nucleoprotein.

Submitted on June 22, 1963


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