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The Journal of Cell Biology, Vol 22, 505-513, Copyright © 1964 by Rockefeller University Press

ARTICLE

CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

: III. Further Studies on the Nature of the DNA-Containing Elements



D. R. Wolstenholme Ph.D.1 and W. Plaut Ph.D.1

1 From the Department of Zoology, University of Wisconsin, Madison, Wisconsin.

Dr. Wolstenholme's present address is Genetisch Instituut der Rijksuniversiteit te Groningen, Haren, The Netherlands

The application of electron microscope autoradiography to Amoeba proteus cells labeled with tritiated thymidine has permitted the identification of morphologically distinct particles in the cytoplasm as the sites of incorporated DNA precursor. The particles correspond to those previously described from light microscope studies, with respect to both H3Tdr incorporation and distribution in centrifugally stratified amoebae. Ingested bacteria differ from the particles, in morphology as well as in the absence of associated label. Attempts to introduce a normal particle labeling pattern by incubating amoebae with labeled sediment derived from used amoeba medium failed. The resultant conclusion, that the particles are maintained in the amoeba by self-duplication, is supported by the presence of particles in configurations suggestive of division.

Submitted on November 7, 1963


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