PRACTICAL STEREOLOGICAL METHODS FOR MORPHOMETRIC CYTOLOGY

doi:10.1083/jcb.30.1.23

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PRACTICAL STEREOLOGICAL METHODS FOR MORPHOMETRIC CYTOLOGY

Ewald R. Weibel ¹, Gonzague S. Kistler ¹, and Walter F. Scherle ¹

¹ From the Department of Anatomy, University of Zürich, Zürich, Switzerland

Stereological principles provide efficient and reliable toolsfor the determination of quantitative parameters of tissue structureon sections. Some principles which allow the estimation of volumetricratios, surface areas, surface-to-volume ratios, thicknessesof tissue or cell sheets, and the number of structures are reviewedand presented in general form; means for their practical applicationin electron microscopy are outlined. The systematic and statisticalerrors involved in such measurements are discussed.

Submitted on December 2, 1965

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