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The Journal of Cell Biology, Vol 34, 219-227, Copyright © 1967 by Rockefeller University Press

ARTICLE

PHAGOCYTOSIS OF LATEX BEADS BY ACANTHAMOEBA

: II. Electron Microscopic Study of the Initial Events



Edward D. Korn 1 and Robert A. Weisman 1

1 From the Laboratory of Biochemistry, the Section on Cellular Physiology, the National Heart Institute, the National Institutes of Health, the Public Health Service, United States Department of Health, Education and Welfare, Bethesda, Maryland 20014

Electron microscopic studies confirm and extend the conclusions derived previously from a quantitative biochemical study of the phagocytosis of polystyrene and polyvinyltoluene latex beads by Acanthamoeba (1). Latex beads 1.305, 1.90, and 2.68 µ in diameter are ingested individually, with each bead tightly surrounded by a membrane derived from the plasma membrane. Latex beads 0.557, 0.264, 0.126, and 0.088 µ in diameter are accumulated at the surface of the ameba and then phagocytosed, with many beads tightly packed within one membrane-bounded vesicle.

Submitted on December 10, 1966


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