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The Journal of Cell Biology, Vol 35, 445-453, Copyright © 1967 by Rockefeller University Press

ARTICLE

THE FINE STRUCTURE OF EMBRYONIC CHICK SKELETAL MUSCLE CELLS DIFFERENTIATED IN VITRO



Y. Shimada 1, D. A. Fischman 1, and A. A. Moscona 1

1 From the Department of Zoology, the University of Chicago, Chicago, Illinois 60637,

Dr. Shimada's permanent address is the Department of Anatomy, School of Medicine, Chiba University, Chiba, Japan. Dr. Fischman's present address is the Armed Forces Institute of Pathology, Washington, D. C. 20012

Dissociated myoblasts from 12-day chick embryos were cultured in monolayer, and the differentiation of skeletal muscle cells was studied by electron microscopy. The results have revealed a striking ultrastructural similarity between the in vivo and the in vitro developing muscle, particularly with respect to the myofibrils and sarcoplasmic reticulum. This study demonstrates that all the characteristic organelles of mature skeletal muscle can develop in vitro in the absence of nerves.

Submitted on March 17, 1967
Revised on July 19, 1967
Accepted on July 19, 1967


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