ARTICLE

A QUANTITATIVE STEREOLOGICAL DESCRIPTION OF THE ULTRASTRUCTURE OF NORMAL RAT LIVER PARENCHYMAL CELLS

¹ From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York 10032

The principles of stereology have been applied to a morphometric analysis of parenchymal cells from the peripheral, midzonal, and central regions of normal rat liver lobules. The fractional volumes of cytoplasm occupied by mitochondria, peroxisomes, lysosomes, lipid, and glycogen have been determined. The surface densities of smooth- and rough-surfaced endoplasmic reticulum and of mitochondrial envelope and cristae have also been measured. The average number and dimensions of mitochondria and peroxisomes have been evaluated. By the use of an independent measurement of the average cytoplasmic volume, these data have been expressed as the actual volumes, areas, and numbers per cell in the different parts of the hepatic lobule. Similarly, the volumes of the envelope, cristae, and matrix compartments and the area of cristae membranes have been calculated for the average-sized mitochondrion in each lobular zone. Structural homogeneity is found in over 80% of normal rat liver parenchymal cells, with most of the significant differences being confined to those cells immediately surrounding the central veins.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Lambert, A. J., Merry, B. J. (2005). Lack of Effect of Caloric Restriction on Bioenergetics and Reactive Oxygen Species Production in Intact Rat Hepatocytes. Journals of Gerontology Series A: Biological Sciences and Medical Sciences 60: 175-180 [Abstract] [Full Text]  
• Burkhardt, S., Bahnemann, R., Failing, K., Reinacher, M. (2004). Zonal Distribution of Cell Proliferation in the Liver of Untreated B6C3F1 and C57BL Mice. Toxicol Pathol 32: 100-105 [Abstract]  
• Peachman, K. K., Lyles, D. S., Bass, D. A. (2001). Mitochondria in eosinophils: Functional role in apoptosis but not respiration. Proc. Natl. Acad. Sci. USA 98: 1717-1722 [Abstract] [Full Text]  
• Anversa, P. (2000). Myocyte Death in the Pathological Heart. Circ. Res. 86: 121-124 [Full Text]  
• Harper, M.-E., Monemdjou, S., Ramsey, J. J., Weindruch, R. (1998). Age-related increase in mitochondrial proton leak and decrease in ATP turnover reactions in mouse hepatocytes. Am. J. Physiol. Endocrinol. Metab. 275: E197-E206 [Abstract] [Full Text]  
• De La Iglesia, F. A., Gray, R. H., Mcguire, E. J. (1992). Subcellular Organelle Biogenesis and Dynamics in Peroxisome Proliferation. International Journal of Toxicology 11: 343-348 [Abstract]  
• Friedman, H. I., Caldwell, M. D., Lollini, L., Shearer, J., Nemeth, T. J. (1982). Effects of Essential Fatty Acid Deficiency on the Induction of Liver Microsomal Membranes by Phenobarbital: An Ultrastructural and Biochemical Investigation. JPEN J Parenter Enteral Nutr 6: 12-19 [Abstract]  
• Schumucker, D., Mooney, J., Jones, A. (1977). Age-related changes in the hepatic endoplasmic reticulum: a quantitative analysis. Science 197: 1005-1008 [Abstract]  
• SUTHERLAND, L. R., EDWARDS, L. A., MEDLINE, A., WILKINSON, R. W., CONNON, J. J. (1977). Meglumine lodipamide (Cholografin(R)) Hepatotoxicity. ANN INTERN MED 86: 437-439 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents