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The Journal of Cell Biology, Vol 38, 151-157, Copyright © 1968 by Rockefeller University Press

ARTICLE

SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS



N. Steele Scott 1, Vinod C. Shah 1, and Robert M. Smillie 1

1 From the Plant Physiology Unit, Commonwealth Scientific and Industrial Research Organization, Division of Food Preservation, Ryde, Australia, and School of Biological Sciences, The University of Sydney, Sydney, Australia.

Dr. Shah's present address is the Department of Zoology, University of Delhi, Delhi - 7, India.

Chloroplasts isolated from Euglena gracilis incorporated both tritiated thymidine 5'-triphosphate and tritiated deoxyadenosine 5'-triphosphate into an acid-stable fraction. The incorporation was dependent on the presence of all four deoxynucleoside triphosphates and was sensitive to treatment with deoxyribonuclease and actinomycin D. It was demonstrated that bacterial contamination could not account for the incorporation of label. Extraction of DNA from the chloroplasts and subsequent density gradient centrifugation of the DNA in CsCl2 showed that the incorporation was into chloroplast DNA (rho = 1.686) of high molecular weight.

Submitted on January 26, 1968
Revised on February 23, 1968


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