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The Journal of Cell Biology, Vol 41, 124-132, Copyright © 1969 by Rockefeller University Press

ARTICLE

PLASMA MEMBRANES OF THE RAT LIVER

: Isolation and Enzymatic Characterization of a Fraction Rich in Bile Canaliculi



C. S. Song 1, W. Rubin 1, A. B. Rifkind 1, and A. Kappas 1

1 From The Rockefeller University and the Departments of Medicine and Anatomy, Cornell University Medical College, New York 10021

A method is described for the rapid isolation of a plasma membrane fraction containing a high concentration of intact bile canaliculi from the rat liver. Isolated bile canaliculi retain most of the ultrastructural features exhibited in the intact liver cell. The final fraction contains 5'-nucleotidase activity at approximately the same concentration as that in previous preparations of plasma membranes. In the presence of 0.01 M Mg++, 5'-nucleotidase exhibits a double pH optimum at pH values of 7.5 and 9.5. The activities of glucose-6-phosphatase and alkaline phosphatase are present in low amounts. Cytochrome P-450 is not detectable. Na+-K+-activation of ATPase is observed to the extent of 20–36% in about half of the assays. The availability of a method for preparation of intact bile canaliculi should prove useful for studying the biochemical events associated with the transport of bile constituents into canaliculi.

Submitted on July 18, 1968
Revised on November 12, 1968


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