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The Journal of Cell Biology, Vol 41, 145-153, Copyright © 1969 by Rockefeller University Press

ARTICLE

EFFECTS OF DIVISION-SYNCHRONIZING HYPOXIC AND HYPERTHERMIC SHOCKS UPON TETRAHYMENA RESPIRATION AND INTRACELLULAR ATP CONCENTRATION



David W. Rooney 1 and John J. Eiler 1

1 From the Department of Biochemistry, University of California Medical Center, San Francisco, California 94122.

Dr. Rooney's present address is the Department of Biology, Saint Louis University, St. Louis, Missouri 63104

The division of Tetrahymena pyriformis GL cells was synchronized with either seven hypoxic or five hyperthermic (heat) shocks. Hyperthermic shocks of 34°C produced no reduction in respiration rate and only a 19% decline in intracellular ATP concentration. Hypoxic shocks of 0.15% ambient oxygen concentration depressed intracellular ATP concentration 50%. It therefore appears that hypoxic shock, but not hyperthermic shock, reverses progress of Tetrahymena toward fission by reducing ATP concentration through a reduction of the rate of oxidative phosphorylation. After the first synchronized division, whether synchronized by intermittent hypoxia or hyperthermia, total respiration rate increased exponentially at the same rate of increase as total respiration rate in an exponentially growing (log phase) Tetrahymena cell culture. Before the first synchronized division, the total respiration rate increased exponentially but more slowly than after completion of the first synchronized division. The pattern of increase of total respiration during division synchronized by either procedure was different than the pattern of increase of total respiration of synchronous cells observed by Zeuthen.

Submitted on July 24, 1968
Revised on November 12, 1968


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