ARTICLE

RIBOFLAVIN AND MOUSE HEPATIC CELL STRUCTURE AND FUNCTION

Bernard Tandler ¹, Robert A. Erlandson ¹, Archie L. Smith ¹, and Ernest L. Wynder ¹

¹ From the Divisions of Cytology, Biochemistry, and Environmental Cancerigenesis, Sloan-Kettering Institute for Cancer Research, New York 10021, and the School of Dentistry, Case Western Reserve University, Cleveland, Ohio 44106.

Dr. Tandler's present address is School of Dentistry, Case Western Reserve University, Cleveland, Ohio 44106. Dr. Smith's present address is Department of Biochemistry, Shulton, Inc., Clifton, New Jersey 07015

Mice which had been on a riboflavin-free diet for 6–8 wk were given daily intraperitoneal injections of riboflavin. The hepatic mitochondria, which in the deficient animals were greatly enlarged, were restored to normal dimensions within 3 days. Normalization of the mitochondrial population was brought about by division of the giant organelles. Dividing mitochondria were characterized by a membranous partition separating the inner compartment into two distinct chambers. Such organelles showed varying degrees of pinching at the level of the partition. The most common site of partition formation was at the base of a small mitochondrial bud. During the 1st day of recovery, dividing mitochondria were so common that they could be easily found in mitochondrial pellets. Injection of riboflavin into normally nourished mice also produced an apparent increase in the frequency of dividing mitochondria in the liver cells.

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