ARTICLE

ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS

¹ From the Departments of Pharmacology and Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, and the Veterans Administration Hospital, West Haven, Connecticut.

Dr. Rajalakshmi's present address is Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania. Dr. Adams's present address is Department of Pathology, University of Florida College of Medicine, Gainesville, Florida

Centrifugation of a sucrose homogenate of the livers of female albino rats fed a 1.5% orotic acid diet for 3 wk yielded a pellicle containing low density structures. In morphology and biochemical properties these structures resembled those portions of endoplasmic reticulum which accumulated lipid. Electron microscopy indicated large droplets of lipid bounded by a membrane with attached ribosome-like particles. The presence of ribosomes in these structures was established by treatment with deoxycholate and centrifugation. The proportion of 18S and 29S RNA was the same as that found in the ribosomes from normal liver; however, the distribution of radioactivity between the 18S and the 29S RNA after injection of 8-¹⁴C-adenine was distinctly different. The RNA isolated from these structures contained a higher guanylic acid to cytidylic acid ratio than that found in the microsomes of the normal liver. It is proposed that these low density structures may be those portions of the endoplasmic reticulum in which there exists a defect responsible for the block in the assembly or secretion of plasma lipoprotein.

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