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The Journal of Cell Biology, Vol 43, 80-89, Copyright © 1969 by Rockefeller University Press

ARTICLE

OSMIUM ZINC IODIDE REACTIVE SITES IN THE EPIDERMAL LANGERHANS CELL



Gustav Niebauer 1, Walter S. Krawczyk 1, Richard L. Kidd 1, and George F. Wilgram 1

1 From the Dermatologic Genetics Laboratory, Tufts New England Medical Center Hospital, Boston, Massachusetts 02111, and the Department of Oral Histopathology, Harvard School of Dental Medicine, Boston, Massachusetts 02114

Fixation of epidermis with a mixture of osmium tetroxide and zinc iodide (OsO4-ZnI2) for 24 hr renders the central periodic lamella of the Langerhans cell granule (LCG), the Golgi region, and the nuclear envelope of epidermal Langerhans cells preferentially visible. The use of this technique on Langerhans cells in normal epidermis and in epidermis of patients with histiocytosis (Letterer-Siwe disease) allows a broader visualization of the LCG's than was heretofore possible with routine glutaraldehyde-osmium tetroxide fixation and uranyl acetate-lead staining. The identical staining of Golgi apparatus and LCG favors the view that there is close relation between the Golgi area and the LCG's. Different staining characteristics of the LCG's near the Golgi region and at the cell periphery, respectively, may suggest that the LCG undergoes changes on its way from the Golgi area towards the extracellular space. The hypothesis is advanced that the material which is heavily impregnated with metal after fixation with OsO4-ZnI2 might be a lipid.

Submitted on February 24, 1969
Revised on May 7, 1969


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