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The Journal of Cell Biology, Vol 50, 222-231, Copyright © 1971 by Rockefeller University Press

ARTICLE

MORPHOLOGY AND FUNCTION OF CELLS OF HUMAN EMBRYONIC LIVER IN MONOLAYER CULTURE



D. Montgomery Bissell 1 and Jeremiah G. Tilles 1

1 From the Thorndike Memorial Laboratory, Harvard Medical Unit, Boston City Hospital 02118, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115

A system for culturing human fetal liver cells in monolayers is described and the effects of various conditions of growth on the morphology and function of the cultured cells are presented. The addition of 10% calf serum or 1% human serum to the growth medium accelerated the proliferation of the liver cells, with subsequent loss of characteristic morphology and specific functional activity. In the absence of serum, the cultured liver cells retained their morphology and their function for at least 4 wk, as evidenced by secretion of serum albumin and storage of glycogen and iron.

Submitted on September 2, 1970
Revised on January 7, 1971


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