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The Journal of Cell Biology, Vol 59, 633-642, Copyright © 1973 by Rockefeller University Press

ARTICLE

A SCANNING ELECTRON MICROSCOPE STUDY OF SURFACE FEATURES OF VIRAL AND SPONTANEOUS TRANSFORMANTS OF MOUSE BALB/3T3 CELLS



Keith R. Porter 1, George J. Todaro 1, and Virginia Fonte 1

1 From the Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80302, and National Cancer Institute, Bethesda, Maryland 20014.

Dr. Todaro is from the National Cancer Institute.

Cells of the mouse line Balb/3T3 as well as three virus-induced transformants and two spontaneous transformants grown in vitro have been studied for their topography by scanning electron microscopy. The parent cell in confluent culture closely resembles an endothelial cell in its form and in the structure of its association with adjacent cells. The tumorigenic transformants produced by SV40, murine sarcoma virus, or polyoma viruses are fusiform to pleomorphic and distinctly different from the cell of origin. They show relatively smooth surfaces except for blebs and marginal microvilli. Perhaps most surprising is the similarity they bear to one another. This is made the more singular by the very different form shown by the tumorigenic transformants of spontaneous origin. One of these, S2-4, possesses a thickened rather than the lamellar form of the parent A31 cell and is covered by long microvilli and many spherical blebs. The other, TuT3, more closely resembles the cell of origin but shows extensive ruffling at its margins. All transformants grow without evidence of contact inhibition.

The significance of the surface morphologies and the factors influencing cell form are discussed.

Submitted on June 18, 1973
Revised on July 13, 1973


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