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The Journal of Cell Biology, Vol 60, 26-38, Copyright © 1974 by Rockefeller University Press

ARTICLE

CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA



Howard J. Allen 1, Charles Ault 1, Richard J. Winzler 1, and James F. Danielli 1

1 From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

Dr. Alien's present address is the Department of Gynecology, Boswell Park Memorial Institute, Buffalo, New York 14203.

The cell surface has been isolated from uninucleate, freshwater, phagocytic amoebae by a new procedure. Several criteria were employed to demonstrate purity of the cell surface fraction. All morphological components of the tripartite surface were present in the isolated surface and the weight of the isolated surface was quantitatively accounted for by the components analyzed. Chemical analyses showed the presence of lipid, protein, and carbohydrate. Mannose was the predominant neutral sugar. Analyses for three different strains of Amoeba were similar. Phosphate was found to be the major anionic group in the cell surface material. Sulfate, uronic acid, sialic acid, muramic acid, and nonamidated glutamic acid and aspartic acid were absent. Evidence is presented suggesting that the phosphate is associated with an unidentified nonreducing polyol.

Submitted on November 27, 1972
Revised on August 20, 1973


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