POROUS SUBSTRUCTURE OF THE GLOMERULAR SLIT DIAPHRAGM IN THE RAT AND MOUSE

doi:10.1083/jcb.60.2.423

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POROUS SUBSTRUCTURE OF THE GLOMERULAR SLIT DIAPHRAGM IN THE RAT AND MOUSE

Richard Rodewald ¹ and Morris J. Karnovsky ¹

¹ From the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.

Dr. Rodewald's present address is the Department of Biology, University of Virginia, Chariottesville, Virginia 22901.

The highly ordered, isoporous substructure of the glomerularslit diaphragm was revealed in rat and mouse kidneys fixed byperfusion with tannic acid and glutaraldehyde. The slit diaphragmwas similar in both animal species and appeared as a continuousjunctional band, 300–450 Å wide, consistently presentwithin all slits formed by the epithelial foot processes. Thediaphragm exhibited a zipper-like substructure with alternating,periodic cross bridges extending from the podocyte plasma membranesto a central filament which ran parallel to and equidistantfrom the cell membranes. The dimensions and spacing of the crossbridges defined a uniform population of rectangular pores approximately40 by 140 Å in cross section and 70 Å in length.The total area of the pores was calculated to be about 2–3%of the total surface area of the glomerular capillaries. Physiologicaldata indicate that the glomerular filter functions as if itwere an isoporous membrane which excludes proteins larger thanserum albumin. The similarity between the dimensions of thepores in the slit diaphragm and estimates for the size and shapeof serum albumin supports the conclusion from tracer experimentsthat the slit diaphragm may serve as the principal filtrationbarrier to plasma proteins in the kidney.

Submitted on August 20, 1973
Revised on October 9, 1973

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Rippe, B. (2004). What is the role of albumin in proteinuric glomerulopathies?. Nephrol Dial Transplant 19: 1-5 [Full Text]
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Barletta, G.-M., Kovari, I. A., Verma, R. K., Kerjaschki, D., Holzman, L. B. (2003). Nephrin and Neph1 Co-localize at the Podocyte Foot Process Intercellular Junction and Form cis Hetero-oligomers. J. Biol. Chem. 278: 19266-19271 [Abstract] [Full Text]
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Gerke, P., Huber, T. B., Sellin, L., Benzing, T., Walz, G. (2003). Homodimerization and Heterodimerization of the Glomerular Podocyte Proteins Nephrin and NEPH1. J. Am. Soc. Nephrol. 14: 918-926 [Abstract] [Full Text]
Kawachi, H., Koike, H., Kurihara, H., Sakai, T., Shimizu, F. (2003). Cloning of Rat Homologue of Podocin: Expression in Proteinuric States and in Developing Glomeruli. J. Am. Soc. Nephrol. 14: 46-56 [Abstract] [Full Text]
Pavenstadt, H., Kriz, W., Kretzler, M. (2003). Cell Biology of the Glomerular Podocyte. Physiol. Rev. 83: 253-307 [Abstract] [Full Text]
Mundel, P., Shankland, S. J. (2002). Podocyte Biology and Response to Injury. J. Am. Soc. Nephrol. 13: 3005-3015 [Full Text]
Yaoita, E., Yao, J., Yoshida, Y., Morioka, T., Nameta, M., Takata, T., Kamiie, J.-i., Fujinaka, H., Oite, T., Yamamoto, T. (2002). Up-Regulation of Connexin43 in Glomerular Podocytes in Response to Injury. Am. J. Pathol. 161: 1597-1606 [Abstract] [Full Text]
Hamano, Y., Grunkemeyer, J. A., Sudhakar, A., Zeisberg, M., Cosgrove, D., Morello, R., Lee, B., Sugimoto, H., Kalluri, R. (2002). Determinants of Vascular Permeability in the Kidney Glomerulus. J. Biol. Chem. 277: 31154-31162 [Abstract] [Full Text]
Palmen, T., Lehtonen, S., Ora, A., Kerjaschki, D., Antignac, C., Lehtonen, E., Holthofer, H. (2002). Interaction of Endogenous Nephrin and CD2-Associated Protein in Mouse Epithelial M-1 Cell Line. J. Am. Soc. Nephrol. 13: 1766-1772 [Abstract] [Full Text]
Reiser, J., von Gersdorff, G., Simons, M., Schwarz, K., Faul, C., Giardino, L., Heider, T., Loos, M., Mundel, P. (2002). Novel concepts in understanding and management of glomerular proteinuria. Nephrol Dial Transplant 17: 951-955 [Full Text]
Huh, W., Kim, D. J., Kim, M.-K., Kim, Y. G., Oh, H.-Y., Ruotsalainen, V., Tryggvason, K. (2002). Expression of nephrin in acquired human glomerular disease. Nephrol Dial Transplant 17: 478-484 [Abstract] [Full Text]
Miner, J. H. (2002). Focusing on the Glomerular Slit Diaphragm : Podocin Enters the Picture. Am. J. Pathol. 160: 3-5 [Full Text]
Roselli, S., Gribouval, O., Boute, N., Sich, M., Benessy, F., Attie, T., Gubler, M.-C., Antignac, C. (2002). Podocin Localizes in the Kidney to the Slit Diaphragm Area. Am. J. Pathol. 160: 131-139 [Abstract] [Full Text]
Shih, N.-Y., Li, J., Cotran, R., Mundel, P., Miner, J. H., Shaw, A. S. (2001). CD2AP Localizes to the Slit Diaphragm and Binds to Nephrin via a Novel C-Terminal Domain. Am. J. Pathol. 159: 2303-2308 [Abstract] [Full Text]
Huber, T. B., Kottgen, M., Schilling, B., Walz, G., Benzing, T. (2001). Interaction with Podocin Facilitates Nephrin Signaling. J. Biol. Chem. 276: 41543-41546 [Abstract] [Full Text]
Deen, W. M., Lazzara, M. J., Myers, B. D. (2001). Structural determinants of glomerular permeability. Am. J. Physiol. Renal Physiol. 281: F579-F596 [Abstract] [Full Text]
Ruotsalainen, V., Patrakka, J., Tissari, P., Reponen, P., Hess, M., Kestila, M., Holmberg, C., Salonen, R., Heikinheimo, M., Wartiovaara, J., Tryggvason, K., Jalanko, H. (2000). Role of Nephrin in Cell Junction Formation in Human Nephrogenesis. Am. J. Pathol. 157: 1905-1916 [Abstract] [Full Text]
REISER, J., KRIZ, W., KRETZLER, M., MUNDEL, P. (2000). The Glomerular Slit Diaphragm Is a Modified Adherens Junction. J. Am. Soc. Nephrol. 11: 1-8 [Abstract] [Full Text]
TRYGGVASON, K. (1999). Unraveling the Mechanisms of Glomerular Ultrafiltration: Nephrin, aKey Component of the Slit Diaphragm. J. Am. Soc. Nephrol. 10: 2440-2445 [Full Text]
Edwards, A., Daniels, B. S., Deen, W. M. (1999). Ultrastructural model for size selectivity in glomerular filtration. Am. J. Physiol. Renal Physiol. 276: F892-F902 [Abstract] [Full Text]
Kawachi, H., Kurihara, H., Topham, P. S., Brown, D., Shia, M. A., Orikasa, M., Shimizu, F., Salant, D. J. (1997). Slit diaphragm-reactive nephritogenic MAb 5-1-6�alters expression of ZO-1 in rat podocytes. Am. J. Physiol. Renal Physiol. 273: F984-F993 [Abstract] [Full Text]
Seiler, M., Venkatachalam, M., Cotran, R. (1975). Glomerular epithelium: structural alterations induced by polycations. Science 189: 390-393 [Abstract]
Yuan, H., Takeuchi, E., Salant, D. J. (2002). Podocyte slit-diaphragm protein nephrin is linked to the actin cytoskeleton. Am. J. Physiol. Renal Physiol. 282: F585-F591 [Abstract] [Full Text]