JCB logo
amgmicro.com
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 1210K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wolk, C. P.
Right arrow Articles by Galonsky, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wolk, C. P.
Right arrow Articles by Galonsky, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
The Journal of Cell Biology, Vol 61, 440-453, Copyright © 1974 by Rockefeller University Press

ARTICLE

AUTORADIOGRAPHIC LOCALIZATION OF 13N AFTER FIXATION OF 13N-LABELED NITROGEN GAS BY A HETEROCYST-FORMING BLUE-GREEN ALGA



C. Peter Wolk 1, Sam M. Austin 1, John Bortins 1, and Aaron Galonsky 1

1 From the MSU/AEC Plant Research Laboratory, and the Cyclotron Laboratory and the Department of Physics, Michigan State University, East Lansing, Michigan 48824.

Mr. Bortins' present address is the School of Physics and Astronomy, Tate Laboratory, University of Minnesota, Minneapolis, Minnesota 55414.

13N, generated by proton bombardment of 13C powder, is rapidly and easily converted to 13N-N2, 0.01 atm pressure, ca. 10 mCi/ml, by automated Dumas combustion. 13N fixed (as 13N-N2) by algal filaments was localized by an autoradiographic technique which permits track autoradiography with isotopes having short half-lives. Our findings show directly that a minimum of about 25% of the N2 fixation by intact, aerobically grown filaments of Anabaena cylindrica is carried out by the heterocysts. If all of the N2 fixation takes place in the heterocysts, then the movement of nitrogen along the filaments can be characterized by a constant tau < ca. 5 s (cell-2).

Submitted on June 25, 1973
Revised on October 23, 1973


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents