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The Journal of Cell Biology, Vol 61, 701-722, Copyright © 1974 by Rockefeller University Press

ARTICLE

ELECTRON MICROSCOPE AND EXPERIMENTAL INVESTIGATIONS OF THE NEUROFILAMENTOUS NETWORK IN DEITERS' NEURONS

: Relationship with the Cell Surface and Nuclear Pores



J. Metuzals 1 and W. E. Mushynski 1

1 From the Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, University of Ottawa, Ottawa, Canada and the Institute of Neurobiology, Faculty of Medicine, University of Göteborg, Goteborg, Sweden.

Dr. Mushynski's present address is Department of Biochemistry, McGill University, Montreal 101, Canada.

The assembly of filamentous elements and their relations to the plasma membrane and to the nuclear pores have been studied in Deiters' neurons of rabbit brain. Electron microscopy of thin sections and of ectoplasm spread preparations have been integrated with physicochemical experiments and differential interference microscopy of freshly isolated cells. A neurofilamentous network extends as a continuous, three-dimensional, semilattice structure throughout the ectoplasm, the "plasma roads," and the perinuclear zone of the perikaryon. This space network consists of sim90-Å wide neurofilaments arranged in fascicles which are interconnected by an exchange of neurofilaments. The neurofilaments consist of intercoiled sim20-Å wide unit-filaments and are associated through cross-associating filaments with other neurofilaments of the fascicle and with microfilaments. The sim20–50-Å wide microfilaments display intimate associations with the plasma membrane and with the nuclear pores. Electron microscopy of thin sections from glycerinated and heavy meromyosin-treated Deiters' neurons shows that actin-like filaments are present in the pre- and postsynaptic regions of synapses terminating on these neurons.

It is proposed that the neurofilamentous space network serves a transducing function by linking plasma membrane activities with the genetic machinery of the neuron.

Submitted on July 16, 1973
Revised on January 21, 1974


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