The Journal of Cell Biology, Vol 64, 331-339, Copyright © 1975 by The Rockefeller University Press

ARTICLES

Two improved methods for preparing ferritin-protein conjugates for electron microscopy

Y Kishida, BR Olsen, RA Berg and DJ Prockop

Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin- antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.
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